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Fluorescent nanoscopy method

DC
  • US 8,334,143 B2
  • Filed: 02/06/2012
  • Issued: 12/18/2012
  • Est. Priority Date: 05/18/2005
  • Status: Expired due to Fees
First Claim
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1. A method for 3D microscopy of objects, comprising:

  • introducing a fluorescent agent within an object;

    exciting the fluorescent agent;

    capturing with at least one camera and a data processor a plurality of image frames including separately observed fluorescing spots produced by the excited fluorescent agent;

    saving the captured image frames to a recordable medium associated with the data processor;

    obtaining more than one intensity distribution for each fluorescing spot from different focal planes with a light dividing prism and the at least one camera; and

    constructing a three-dimensional image of the object using differences between the light intensity distributions of each spot detected between the different focal planes.

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